abstract：:Proton transport-coupled unisite catalysis was measured with the H(+)-ATPase from chloroplasts. The reaction was measured in the ATP hydrolysis direction under deenergized conditions and in the ATP synthesis direction under energized conditions. The equilibrium constant of the enzyme does not change upon energization,...

journal_title：Journal of bioenergetics and biomembranes

authors： Gräber P,Labahn A

更新日期：1992-10-01 00:00:00

abstract：:Vacuolar H+ ATPases participate in renal hydrogen ion secretion in both the proximal and distal nephron. These plasma membrane forms of the vacuolar H+ ATPase are regulated physiologically to maintain the acid-base balance of the organism. Proton transporting renal cells have requirements for constitutive acidificatio...

journal_title：Journal of bioenergetics and biomembranes

authors： Gluck SL

更新日期：1992-08-01 00:00:00

abstract：:First, halorhodopsin is capable of pumping protons after illumination with green and blue light in the same direction as chloride. Second, mutated bacteriorhodopsin where the proton acceptor Asp85 and the proton donor Asp96 are replaced by Asn showed proton pump activity after illumination with blue light in the same ...

journal_title：Journal of bioenergetics and biomembranes

authors： Oesterhelt D,Tittor J,Bamberg E

更新日期：1992-04-01 00:00:00

abstract：:Porin or voltage-dependent anion-selective channel (VDAC) is the main protein responsible for the high permeability of the outer mitochondrial membrane. The mitochondrial porin is mainly composed of sided beta-strands, in analogy with bacterial porin, whose structure has been resolved at 1.8 A resolution. In mitochond...

journal_title：Journal of bioenergetics and biomembranes

authors： De Pinto VD,Palmieri F

更新日期：1992-02-01 00:00:00

abstract：:Three classes of inner mitochondrial membrane (IMM) channel activities have been defined by direct measurement of conductance levels in membranes with patch clamp techniques in 150 mM KCl. The "107 pS activity" is slightly anion selective and voltage dependent (open with matrix positive potentials). "Multiple conducta...

journal_title：Journal of bioenergetics and biomembranes

authors： Kinnally KW,Antonenko YN,Zorov DB

更新日期：1992-02-01 00:00:00

abstract：:Transformation of 3T3 cells by SV40 virus changes the properties of the transplasma membrane electron transport activity which can be assayed by reduction of external ferric salts. After 42 h of culture and before the growth rate is maximum, the transformed cells have a much slower rate of ferric reduction. The change...

journal_title：Journal of bioenergetics and biomembranes

authors： Löw H,Crane FL,Grebing C,Isaksson M,Lindgren A,Sun IL

更新日期：1991-12-01 00:00:00

abstract：:The term "direct-coupled" is considered in the context of redox-linked proton translocation mechanisms, and the origins of this concept, its philosophical implications, applications, and contributions to the development of bioenergetics, are discussed. ...

journal_title：Journal of bioenergetics and biomembranes

authors： West IC

更新日期：1991-10-01 00:00:00

abstract：:Protein phosphorylation is an important mechanism in the modulation of voltage-dependent ionic channels. In squid giant axons, the potassium delayed rectifier channel is modulated by an ATP-mediated phosphorylation mechanism, producing important changes in amplitude and kinetics of the outward current. The characteris...

journal_title：Journal of bioenergetics and biomembranes

authors： Perozo E,Bezanilla F

更新日期：1991-08-01 00:00:00

abstract：:The presence of transplasma membrane electron transport in a variety of plant cells and tissues is reported. It is now agreed that this property of eukaryotic cells is of ubiquitous nature. Studies with highly purified plasma membranes have established the presence of electron transport enzymes. Two types of activitie...

journal_title：Journal of bioenergetics and biomembranes

authors： Misra PC

更新日期：1991-06-01 00:00:00

abstract：:The protons excreted by plant cells may arise by two different mechanisms: (1) by the action of the plasma membrane H(+)-ATPase and (2) by plasma membrane redox reactions. The exact proportion from each source is not known, but the plasma membrane H(+)-ATPase is, by far, the major contributor to proton efflux. There i...

journal_title：Journal of bioenergetics and biomembranes

authors： Barr R

更新日期：1991-06-01 00:00:00

abstract：:The presence of additional subunits in cytochrome oxidase distinguish the multicellular eukaryotic enzyme from that of a simple unicellular bacterial enzyme. The number of these additional subunits increases with increasing evolutionary stage of the organism. Subunits I-III of the eukaryotic enzyme are related to the ...

journal_title：Journal of bioenergetics and biomembranes

authors： Kadenbach B,Stroh A,Hüther FJ,Reimann A,Steverding D

更新日期：1991-04-01 00:00:00

abstract：:The chronology of the major discoveries important in elucidating certain aspects of the molecular basis of transmembrane signaling is briefly reviewed. Recent developments linking cell stimulation and transformation are intimated. ...

journal_title：Journal of bioenergetics and biomembranes

authors： Badwey JA

更新日期：1991-02-01 00:00:00

abstract：:A series of amphiphilic polymethlyenecarboxymaleimides has been synthesized for use as sulfhydryl reagents applicable to membrane proteins. Physical properties of the compounds which are relevant to their proposed mode of action have been determined. By comparing rates of reaction in aqueous and aprotic solvents, the ...

journal_title：Journal of bioenergetics and biomembranes

authors： Griffiths DG,Partis MD,Churchill P,Brenner SC,Fleischer S,Moore RJ,Beechey RB

更新日期：1990-10-01 00:00:00

abstract：:Signal peptidases, the endoproteases that remove the amino-terminal signal sequence from many secretory proteins, have been isolated from various sources. Seven signal peptidases have been purified, two from E. coli, two from mammalian sources, and three from mitochondrial matrix. The mitochondrial enzymes are soluble...

journal_title：Journal of bioenergetics and biomembranes

authors： Dev IK,Ray PH

更新日期：1990-06-01 00:00:00

abstract：:In this review, I focus on the bioenergetics of the methanogenic bacteria, with particular attention directed to the roles of transmembrane electrochemical gradients of sodium and proton. In addition, the mechanism of coupling ATP synthesis to methanogenic electron transfer is addressed. Evidence is reviewed which sug...

journal_title：Journal of bioenergetics and biomembranes

authors： Lancaster JR Jr

更新日期：1989-12-01 00:00:00

abstract：:Extremely alkalophilic bacteria that grow optimally at pH 10.5 and above are generally aerobic bacilli that grow at mesophilic temperatures and moderate salt levels. The adaptations to alkalophily in these organisms may be distinguished from responses to combined challenges of high pH together with other stresses such...

journal_title：Journal of bioenergetics and biomembranes

authors： Krulwich TA,Guffanti AA

更新日期：1989-12-01 00:00:00

abstract：:The fungal plasma membrane contains a proton-translocating ATPase that is closely related, both structurally and functionally, to the [Na+, K+]-, [H+, K+]-, and [Ca2+]-ATPases of animal cells, the plasma-membrane [H+]-ATPase of higher plants, and several bacterial cation-transporting ATPases. This review summarizes cu...

journal_title：Journal of bioenergetics and biomembranes

authors： Nakamoto RK,Slayman CW

更新日期：1989-10-01 00:00:00

abstract：:Reconstitution experiments were performed on lipid bilayer membranes in the presence of purified mitochondrial porin from yeast and of detergent-solubilized mitochondrial outer membranes of a porin-free yeast mutant. The addition of the porin resulted in a strong increase of the membrane conductance, which was caused ...

journal_title：Journal of bioenergetics and biomembranes

authors： Benz R,Schmid A,Dihanich M

更新日期：1989-08-01 00:00:00

abstract：:Liver plasma membranes isolated from rats with chronic dietary iron overload showed a large modification of their phospholipid fatty acid composition. Specifically, a significant decrease in polyunsaturated fatty acids and a parallel increase in saturated fatty acids was observed. This pattern was consistent with the ...

journal_title：Journal of bioenergetics and biomembranes

authors： Pietrangelo A,Tripodi A,Carulli N,Tomasi A,Ceccarelli D,Ventura E,Masini A

更新日期：1989-08-01 00:00:00

abstract：:Cytochrome c oxidase was purified from mitochondria of Euglena gracilis and separated into 15 different polypeptide subunits by polyacrylamide gel electrophoresis. All 15 subunits copurify through various purification procedures, and the subunit composition of the isolated enzyme is identical to that of the immunoprec...

journal_title：Journal of bioenergetics and biomembranes

authors： Brönstrup U,Hachtel W

更新日期：1989-06-01 00:00:00

abstract：:Recent studies of isolated muscle membrane have enabled induction and monitoring of rapid Ca2+ release from sarcoplasmic reticulum (SR)5 in vitro by a variety of methods. On the other hand, various proteins that may be directly or indirectly involved in the Ca2+ release mechanism have begun to be unveiled. In this min...

journal_title：Journal of bioenergetics and biomembranes

authors： Ikemoto N,Ronjat M,Mészáros LG

更新日期：1989-04-01 00:00:00

abstract：:The sarcoplasmic reticulum (SR) of skeletal muscle controls the contraction-relaxation cycle by raising and lowering the myoplasmic free-Ca2+ concentration. The coupling between excitation, i.e., depolarization of sarcolemma and transverse tubule (TT) and Ca2+ release from the terminal cisternae (TC) of SR takes place...

journal_title：Journal of bioenergetics and biomembranes

authors： Volpe P

更新日期：1989-04-01 00:00:00

abstract：:Absorbance changes at 446 nm in purified cytochrome oxidase following flash photolysis of carboxy-oxidase poised in the mixed valance state at +220 mV show biphasic kinetics. One phase corresponds to CO recombination to ferrous cytochrome a3 with an energy of activation of 9 kcal/mol; the second phase is 3-5 times fas...

journal_title：Journal of bioenergetics and biomembranes

authors： Harmon HJ

更新日期：1988-12-01 00:00:00

abstract：:A great deal of progress has been made in understanding both the structure and the mechanism of F1-ATPase. The primary structure is now fully known for at least five species. Sequence comparison between chloroplast, photobacteria, aerobic bacteria, and mitochondrial representatives allow us to infer more general funct...

journal_title：Journal of bioenergetics and biomembranes

authors： Ysern X,Amzel LM,Pedersen PL

更新日期：1988-08-01 00:00:00

abstract：:Retinoic acid inhibits the reduction of diferric transferrin through the transplasma membrane electron transport system on fetal rat liver cells infected with a temperature-sensitive SV40 virus when the cells are in the nontransformed state cultured at 40 degrees C. When the cells are in the transformed state (grown a...

journal_title：Journal of bioenergetics and biomembranes

authors： Sun IL,Toole-Simms W,Crane FL,Morré DJ,Löw H,Chou JY

更新日期：1988-06-01 00:00:00

abstract：:Defects in Complex I of the mitochondrial respiratory chain have been identified in 38 patients. The clinical and laboratory features are reviewed and the results of recently devised strategies aimed at characterizing the primary molecular and genetic abnormalities are presented. Although not exhaustive, these studies...

journal_title：Journal of bioenergetics and biomembranes

authors： Morgan-Hughes JA,Schapira AH,Cooper JM,Clark JB

更新日期：1988-06-01 00:00:00

abstract：:Gramicidin A forms ion-conducting channels which can traverse the hydrocarbon core of lipid bilayer membranes. The structures formed by gramicidin A are among the best characterized of all membrane-bound polypeptides or proteins. In this review a brief summary is given of the occurrence, conformation, and synthesis of...

journal_title：Journal of bioenergetics and biomembranes

authors： Cornell B

更新日期：1987-12-01 00:00:00

abstract：:Specific molecular interactions that determine many of the functions of a biomembrane have a high probability of occurring at the surface of that membrane. However, unlike their hydrophobic core, the polar-apolar interface of biomembranes has been somewhat neglected experimentally. Reasons for this are that the chemic...

journal_title：Journal of bioenergetics and biomembranes

authors： Watts A

更新日期：1987-12-01 00:00:00

abstract：:The proton/hydroxide (H+/OH-) permeability of phospholipid bilayer membranes at neutral pH is at least five orders of magnitude higher than the alkali or halide ion permeability, but the mechanism(s) of H+/OH- transport are unknown. This review describes the characteristics of H+/OH- permeability and conductance throu...

journal_title：Journal of bioenergetics and biomembranes

authors： Gutknecht J

更新日期：1987-10-01 00:00:00

abstract：:Mitochondrial cytochrome c (horse), which is a very efficient electron donor to bacterial photosynthetic reaction centers in vitro, binds to the reaction center of Rhodospirillum rubrum with an approximate dissociation constant of 0.3-0.5 microM at pH 8.2 and low ionic strength. The binding site for the reaction cente...

journal_title：Journal of bioenergetics and biomembranes

authors： Bosshard HR,Snozzi M,Bachofen R

更新日期：1987-08-01 00:00:00

abstract：:The induction of millisecond delayed fluorescence mediated by PS I-dependent proton pumping has been used as an indicator of the time course with which those protons equilibrate with sites on the oxygen-evolving enzyme complex (Bowes, J. M., and Crofts, A. R. (1978). Z. Naturforsch. 33C, 271-275). We found that the in...

journal_title：Journal of bioenergetics and biomembranes

authors： Theg SM,Belanger KM,Dilley RA

更新日期：1987-02-01 00:00:00

abstract：:The cytochrome b6/f complex of higher plant chloroplasts is uniformly distributed throughout both appressed and nonappressed thylakoids, in contrast to photosystem II and photosystem I, the other major membrane protein complexes involved in electron transport. We discuss how this distribution is likely to affect inter...

journal_title：Journal of bioenergetics and biomembranes

authors： Allred DR,Staehelin LA

更新日期：1986-10-01 00:00:00

abstract：:Changes in protein and fatty acid compositions of flounder sarcoplasmic reticulum during NADH plus ascorbate-dependent lipid peroxidation in vitro were related to the ability of the sarcoplasmic reticulum to sequester Ca+2. Progressive accumulation of high-molecular-weight protein components occurred concomitantly wit...

journal_title：Journal of bioenergetics and biomembranes

authors： Luo SW,Hultin HO

更新日期：1986-08-01 00:00:00

abstract：:The plasma membrane-enriched fraction isolated from smooth muscle of rat gastric fundus was found to contain a substantial level of potassium-stimulated p-nitrophenylphosphatase activity (K-pNPPase), and its subcellular distribution closely resembled that of other plasma membrane enzyme markers. The kinetic profile of...

journal_title：Journal of bioenergetics and biomembranes

authors： Kostka P,Kwan CY

更新日期：1986-08-01 00:00:00

abstract：:It is proven that any model of localized protonmotive energy coupling that relies upon properties of a homogeneous surface phase must, when operated in the steady state, lead to bulk phase electrochemical potentials for protons that are as large as those required by the delocalized chemiosmotic theory. To obtain model...

journal_title：Journal of bioenergetics and biomembranes

authors： Nagle JF,Dilley RA

更新日期：1986-02-01 00:00:00

abstract：:Beef heart cytochrome c oxidase was reconstituted in asolectin liposomes containing the pH indicator fluorescein-phosphatidylethanolamine (FPE) by the cholate-dialysis procedure. The influence of FPE on the asolectin liposome size and of the removal of subunit III from the complex on its incorporation into liposomes w...

journal_title：Journal of bioenergetics and biomembranes

authors： Müller M,Azzi A

更新日期：1985-12-01 00:00:00

abstract：:Phosphatase activity of a kidney (Na + K)-ATPase preparation was optimally active with Mg2+ plus K+. Mn2+ was less effective and Ca2+ could not substitute for Mg2+. However, adding Ca2+ with Mg2+ or substituting Mn2+ for Mg2+ activated it appreciably in the absence of added K+, and all three divalent cations decreased...

journal_title：Journal of bioenergetics and biomembranes

authors： Robinson JD

更新日期：1985-06-01 00:00:00

abstract：:In order to determine whether polymorphic forms of the Ca2+ + Mg2+-dependent ATPase exist, we have examined the cross-reactivity of five monoclonal antibodies prepared against the rabbit skeletal muscle sarcoplasmic reticulum enzyme with proteins from microsomal fractions isolated from a variety of muscle and nonmuscl...

journal_title：Journal of bioenergetics and biomembranes

authors： Zubrzycka-Gaarn E,MacDonald G,Phillips L,Jorgensen AO,MacLennan DH

更新日期：1984-12-01 00:00:00

abstract：:Calcium may function directly in several aspects of photosynthesis. It appears to modulate activity of the phosphatase enzymes in the carbon reduction cycle and also to regulate chloroplast NAD+ kinase activity through a calmodulin-like protein. Some evidence supports a calcium function in the water-splitting complex,...

journal_title：Journal of bioenergetics and biomembranes

authors： Brand JJ,Becker DW

更新日期：1984-08-01 00:00:00

abstract：:Reaction of a dog kidney (Na + K)-ATPase with pyridoxal phosphate, followed by borohydride reduction, reduced the catalytic activity when measured subsequently. The time course of inactivation did not follow a first-order process, and certain characteristics of the residual enzymatic activity were modified. Moreover, ...

journal_title：Journal of bioenergetics and biomembranes

authors： Robinson JD

更新日期：1984-06-01 00:00:00